The acidification experiment will last for 3 months. In order to study the progress of the acidification impact on the organisms 3 replicates of each organsim/tank will be sampled in a twenty days basis . During this period additional samplings of Hexaplex and Nassarius are planned (every 10 days).
First we measure the length of the studied organisms to record their growth.
|Measurement of a Hexaplex|
Then we choose randomly three samples from every organism and store them in a prelabelled bag.
|Selection of Nassarius|
Then we put them in liquid nitrogen in order to preserve their biofilm and structure intact. Finally we store them at a -80oC freezer.
|Liquid nitrogen container with samples|